Human neural progenitor cells (hNPCs), what are they? What's the connection with Caapi Pure Vine?
hNPCs are a type of stem cell found in the nervous system that have the ability to differentiate into various types of neural cells, including neurons, astrocytes, and oligodendrocytes. They are considered PROMISING for therapeutic applications in treating neurological disorders because of their potential to replace damaged or dysfunctional cells in the brain and spinal cord. Harmine has been expressed in many studies that it stimulates hNPCs.
So if the harmine alkaloid stimulates proliferation of hNPCs, that's sort of a big deal, right? Proliferation is the rapid increase in the number of cells through division, essential for growth, tissue repair, and immune response!
Here's an article from the NCBI: "Harmine stimulates proliferation of human neural progenitors"​
The abstract: "Harmine is the β-carboline alkaloid with the highest concentration in the psychotropic plant decoction Ayahuasca. In rodents, classical antidepressants reverse the symptoms of depression by stimulating neuronal proliferation. It has been shown that Ayahuasca presents antidepressant effects in patients with depressive disorder. In the present study, we investigated the effects of harmine in cell cultures containing human neural progenitor cells (hNPCs, 97% nestin-positive) derived from pluripotent stem cells. After 4 days of treatment, the pool of proliferating hNPCs increased by 71.5%. Harmine has been reported as a potent inhibitor of the dual specificity tyrosine-phosphorylation-regulated kinase (DYRK1A), which regulates cell proliferation and brain development. We tested the effect of analogs of harmine, an inhibitor of DYRK1A (INDY), and an irreversible selective inhibitor of monoamine oxidase (MAO) but not DYRK1A (pargyline). INDY but not pargyline induced proliferation of hNPCs similarly to harmine, suggesting that inhibition of DYRK1A is a possible mechanism to explain harmine effects upon the proliferation of hNPCs. Our findings show that harmine enhances proliferation of hNPCs and suggest that inhibition of DYRK1A may explain its effects upon proliferation in vitro and antidepressant effects in vivo..."​
TLDR: Harmine, a key component of Ayahuasca, has been found to increase the proliferation of human neural progenitor cells (hNPCs) derived from stem cells by 71.5%. This compound inhibits DYRK1A, a kinase that regulates cell proliferation and brain development, with similar effects observed with its analog INDY. However, another analog, pargyline, which does not inhibit DYRK1A, did not induce proliferation. These findings suggest that harmine's promotion of hNPC proliferation and its antidepressant effects may be linked to DYRK1A inhibition, shedding light on its potential therapeutic mechanisms...​
*DYRK: dual-specificity tyrosine phosphorylation-regulated kinase. These kinases play crucial roles in regulating cell proliferation and differentiation across various organisms*
​Source:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6343205/​​​
What is Stem Cell Therapy? What's the relation to Harmine (one of the several main alkaloids of B. Caapi).
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Harmine and Stem Cell Therapy? Harmine stimulates neurogenesis and neural stem cell proliferation! However, where does harmine come from? Well many sources, but one of the primary sources is Banisteriopsis Caapi! It is RICH with harmine alkaloids!!
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The correlation between stem cell therapy and harmine alkaloid lies in harmine's ability to stimulate neural stem cell proliferation, migration, and differentiation. This means that harmine has the potential to promote the growth and development of neural stem cells, which are crucial for repairing and regenerating damaged or dysfunctional neural tissue. Harmine, in essence, initiates faulty cells to die off via apoptosis (cellular self-destruction) through a protein family known as DYRK! The harmine alkaloid may enhance the effectiveness of stem cell therapy by facilitating the generation of new neural cells, hence migration and differentiation, which could be beneficial for treating neurological disorders and injuries.
Here's the article from the NCBI: "STEM CELL THERAPY FOR NEUROLOGICAL DISORDERS: A FOCUS ON AGING."
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The abstract: "Age-related neurological disorders continue to pose a significant societal and economic burden. Aging is a complex phenomenon that affects many aspects of the human body. Specifically, aging can have detrimental effects on the progression of brain diseases and endogenous stem cells. Stem cell therapies possess promising potential to mitigate the neurological symptoms of such diseases. However, aging presents a major obstacle for maximum efficacy of these treatments. In this review, we discuss current preclinical and clinical literature to highlight the interactions between aging, stem cell therapy, and the progression of major neurological disease states such as Parkinson’s disease, Huntington’s disease, stroke, traumatic brain injury, amyotrophic lateral sclerosis, multiple sclerosis, and multiple system atrophy. We raise important questions to guide future research and advance novel treatment options..."
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As we get older, we start to research vitamins and minerals that keep us healthy, but often times, we forget about the functionality and health of our brain. Substances like Ginkgo biloba for our cognition are common, for example. What about Caapi pure vine? The evidence is there, and it is far too underrated.
Source:
Exploring Harmine's Therapeutic Potential: Insights into Cardiac Health, Neurological Disorders, and Stem Cell Therapy.
Harmine (one of the main alkaloids of Banisterdiopsis Caapi) regulates the heart and how it's an anti-inflammatory agent. There's another particular study (cited below) regarding the harmine alkaloid explaining how your brain will clear up bad neuronal cells via "apoptosis," which is self-destruction of a cell.
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Harmine's potential therapeutic effects: Harmine, an alkaloid found in certain plants, inhibits DYRK family proteins and mitogen-activated protein kinase, affecting cell proliferation and apoptosis. Research shows harmine induces apoptosis in neuroblastoma (NB) cell lines, particularly those with amplified MYCN genes. Harmine's interaction with DYRK2 suggests a potential therapeutic approach for NB. Additionally, harmine shows promise in cardiac hypertrophy treatment by inhibiting NF-κB phosphorylation and suppressing inflammatory cytokines. Harmine's potential therapeutic benefits extend to neurological disorders, where stem cell therapy holds promise. Disorders like Alzheimer’s, Parkinson’s, Huntington’s, multiple sclerosis, and amyotrophic lateral sclerosis have diverse treatments that could potentially benefit from stem cell therapy.
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Dual-specificity tyrosine phosphorylation-regulated kinases (DYRKs): These kinases play vital roles in regulating cell proliferation and differentiation across various organisms. They control cell cycle progression by phosphorylating key regulators like cyclins and transcription factors. DYRKs influence protein turnover, either by inducing degradation or stabilization of target proteins. Specific members like DYRK2 have been highlighted for their role in degrading proteins critical for cell cycle progression.
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Since B. Caapi triggers neural stem cell proliferation, migration, and differentiation, it now makes sense how new neural pathways are created! Old pathways (neurons) are forced to die, and new ones are created via Neural Stem Cell Proliferation!
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Every single component of the pure vine helps your whole body! Heart, brain, cell cycle regulation, even apoptosis. This is information that many doctors before us have discovered, and now it's our job to inform everyone! Below are the attached studies I encourage every one of you to read for yourselves!
source:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8724320/
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5992763/